By Tanya E. S. Dahms, Kirk J. Czymmek
The objective of this quantity is to explain the newest advances in microscopic equipment, together with built-in ideas, as utilized to mycology. every one bankruptcy will supply a short evaluation of a selected microscopic process with linked benefits and barriers, the study questions that may be competently addressed utilizing those microscopic equipment, the way it has been effectively utilized to deal with mycological learn questions, together with aiding and complimentary thoughts, and which destiny questions should be addressed.
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Extra info for Advanced Microscopy in Mycology
Plenum Press, New York Pawley JB (2010) Handbook of biological confocal microscopy (Google eBook). Springer, New York Rasmussen CG, Glass NL (2007) Localization of RHO-4 indicates differential regulation of conidial versus vegetative septation in the filamentous fungus Neurospora crassa. Eukaryot Cell 6:1097–1107 Read ND, Hickey PJ (2001) The vesicle trafficking network and tip growth in fungal hyphae. In: Geitmann A, Cresti M, Heath IB (eds) Cell biology of plant and fugal tip growth. IOS Press, Amsterdam Read ND, Kalkman ER (2003) Does endocytosis occur in fungal hyphae?
Available from PM: 19656299) Etxebeste O, Ugalde U, Espeso EA (2010) Adaptative and developmental responses to stress in Aspergillus nidulans. Curr Protein Pept Sci 11(8):704–718. (Available from PM: 21235506) Etxebeste O, Villarino M, Markina-Inarrairaegui A, Araujo-Bazan L, Espeso EA (2013) Cytoplasmic dynamics of the general nuclear import machinery in apically growing syncytial cells. PLoS ONE 8(12):e85076. (Available from PM: 24376868) Fischer R, Timberlake WE (1995) Aspergillus nidulans apsA (anucleate primary sterigmata) encodes a coiled-coil protein required for nuclear positioning and completion of asexual development.
A bright green fluorescent chromophore, generated spontaneously by autocatalysis, can be permanently photoconverted to red with near-UV irradiation. Protein engineering led to monomers of EosFP available and suitable for PALM, namely mEos2, mEosFPthermo, and mIrisFP, the latter of which combines reversible on–off photoactivation switching with irreversible green-to-red photoconversion (McKinney et al. 2009; Wiedenmann et al. 2011). Both proteins have been used in a variety of applications that demonstrate the versatility of these marker proteins in live-cell imaging (Wiedenmann et al.