By Phyllis R. Brown
"Volume forty provides an authoritative choice of the simplest and most recent examine findings in separation technological know-how. Surveys contemporary advancements in excessive performance-liquid (HPLC), reversed-phase liquid (RPLC), countercurrent (CCC), and micellar electrokinetic chromatography (MEKC)."
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This thirty fourth quantity examines topics akin to high-performance capillary electrophoresis; gasoline chromatography, matrix isolation, and infrared spectrometry; and statistical theories of top overlap in chromatography.
"Volume forty provides an authoritative number of the easiest and most modern examine findings in separation technology. Surveys fresh advancements in excessive performance-liquid (HPLC), reversed-phase liquid (RPLC), countercurrent (CCC), and micellar electrokinetic chromatography (MEKC). "
Content material: Acknowledgements -- ch. 1. creation -- ch. 2. research of phosphopeptides via mass spectronomy -- ch. three. Phosphopeptide enrichment -- ch. four. Dephosphorylation -- ch. five. Protein phosphorylation and point mass spectronomy -- ch. 6. Structural phosphorylation research -- ch. 7. Quantitative protein phosphorylation research -- Outlook -- topic index
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Extra resources for Advances in Chromatography, Volume 40
In the method described here, the RP packing material is reacted with an aqueous solution of hydrofluoric acid. The cleavage products are extracted with n-hexane and analyzed by gas chromatography, nuclear magnetic resonance, and mass spectrometry. , of octadecyldimethylchlorosilane (ODDCS) and octadecyldimethylfluorosilane (ODDFS), and the spectra of the reaction products of the chemical cleavage of the ligands of a commercial ODSsilica gel (YMC ODS) with aqueous hydrofluoric acid . The 13C-NMR spectrum of ODDCS was measured with complete decoupling.
6) and (8), the equilibrium constant, K, of a solute in a mobile phase i in equilibrium with the RPLC adsorbent (Ki) can be expressed as follows [29,30]: The contribution of the electrostatic term [the third term on the right-hand side (RHS) of Eq. (8)] to DGliq is assumed to be negligibly small in the case of hydrophobic interactions. According to Eq. , the relative reduction in the hydrophobic surface area caused by adsorption). , a 70% methanol and a 70% acetonitrile solution, respectively).
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