By Alton Meister
Advances in Enzymology and similar parts of Molecular Biology is a seminal sequence within the box of biochemistry, providing researchers entry to authoritative experiences of the newest discoveries in all components of enzymology and molecular biology. those landmark volumes date again to 1941, supplying an unmatched view of the old improvement of enzymology. The sequence bargains researchers the newest figuring out of enzymes, their mechanisms, reactions and evolution, roles in complicated organic approach, and their software in either the laboratory and undefined. each one quantity within the sequence positive factors contributions through prime pioneers and investigators within the box from around the globe. All articles are conscientiously edited to make sure thoroughness, caliber, and clarity.
With its wide variety of themes and lengthy ancient pedigree, Advances in Enzymology and similar components of Molecular Biology can be utilized not just through scholars and researchers in molecular biology, biochemistry, and enzymology, but in addition by way of any scientist attracted to the invention of an enzyme, its houses, and its functions.
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Extra info for Advances in Enzymology and Related Areas of Molecular Biology, Volume 11
3). 42 J. F. DANIELLI BND J. T. DAVIES The main drawbacks t o this use of the Donnan theory are: (1)the activity coefficients are not readily taken into account, (2) the ionic atmosphere lying outside the mean radius is neglected, and (3) pH, is a mean value over a thickness of as much as 30 A. Local variations inside such a large region must occur, but cannot be treated in the simplified form of the theory. Davies and Rideal (23) have examined the concept of a surface phase, 1 / thick, ~ where 1 / is ~ the DebyeHuckel radius, in comparison with a distribution of univalent ions according to the equations of Gouy (29).
38 J. F. DANIELLI AND J. T. DAVIES HA and A’ refer to the undissociated acid and its anion, which are insoluble in the aqueous phase. B’ refers to the concentrations of the anion of the salt used for buffering the aqueous solutions. The Donnan equilibrium gives : and equilibrium between the acid HA and its ions in the interface is given by : [H+la[A’la = KsIHAIs (2) Combining these equations, we obtain : When the acid HA is 50% dissociated at the interface, [A‘], and : = [HA],, By assuming that lowering of the interfacial tension below that of the undissociated acid is proportional to the percentage of dissociation in the surface phase, the amount of dissociation corresponding to any bulk pH value can be found from experiment.
The history of the study of reactions at interfaces is comparatively brief. Thomson (57) was the first to suggest, in 1888, that chemical equilibria at interfaces might be different from those in bulk phases. But the necessary techniques for the study of these phenomena were not sufficiently established until about 1930. By this date studies by Langmuir, Adam, Rideal, and Harkins (3) and their colleagues had thoroughly established the study, on the molecular level, of the physics and chemistry of surfaces.